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Image Search Results
Journal: Cell metabolism
Article Title: Defined Paraventricular Hypothalamic Populations Exhibit Differential Responses to Food Contingent on Caloric State
doi: 10.1016/j.cmet.2018.10.016
Figure Lengend Snippet:
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-c-Fos rabbit Millipore-Sigma ABE457 Anti-c-Fos rabbit Cell Signaling 2250 Anti-GFP chicken Life Technologies A10262 Anti-tdTomato Kerafast EST203 Anti-Oxytocin Peninsula T4084 Laboratories RNAscope probes Mm-GLP1R Advanced Cell Diagnostics 418851 Mm-GLP1R-C2 Advanced Cell Diagnostics 418851-C2 Mm-CRH-C2 Advanced Cell Diagnostics 31609-C2 Mm-MC4R Advanced Cell Diagnostics 402741 Mm_MC4R-C2 Advanced Cell Diagnostics 319181-C2 Bacterial and Virus Strains AAV1-CAG-FLEX-GCaMP6s-WPRE-SV40 AAV-DJ-CMV-DIO-eGFP-2A-TeNT Stanford Viral Core GVVCAAV-71 Chen et al., 2015 Penn Vector Core AV-1-PV2818 pAAV8-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al., 2011 Addgene Viral Core 44361 pAAV8-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al., 2011 Addgene Viral Core 44362 pAAV8-hSyn-dF-HA-KORD-IRES-mCitrine Vardy et al., 2015 Addgene Viral Core 65417 AAV5-EF1a-DIO-EYFP-WPRE-pA Tye et al., 2013 UNC Vector Core AAV-DJ-CMV-DIO-eGFP-2A-TeNT Similar to Campos et al., 2018 Stanford Viral Core GVVCAAV-71 Chemicals, Peptides, and Recombinant Proteins Clozapine-N-Oxide Gift from Bryan Roth N/A Saline Medline DYND1000 Liraglutide Gift from Randy Seeley N/A Ghrelin Abcam ab120231 SALB Gift from Bryan Roth N/A DMSO Sigma-Aldrich 472301 Experimental Models: Organisms/Strains Glp1r-ires-Cre Gift from Steve Liberles JAX_029283 Mc4r-T2a-Cre Gift from Brad Lowell JAX_030759 Oxt-ires-Cre Gift from Brad Lowell JAX_024234 Crh-ires-Cre Gift from Brad Lowell N/A Cg- Gt(ROSA)26Sor tm3(CAG-EYFP) The Jackson Laboratory JAX_007903 Cg- Gt(ROSA)26Sor t63(CAG-ZsGreen) The Jackson Laboratory JAX_007906 Software and
Techniques: RNAscope, Virus, Plasmid Preparation, Recombinant, Saline, Software